119 / 670
Table 5. Suitable range of sample concentration after dilution.
SRM
Product
Code
Reference
value
Sample
size
HPLC-
Injected
Sample
Measured
value
Recovery
vs CoA
Calibration
Standard
Suitable
range
(mg/kg
powder)
(g)
Conc.
(ng/mL)
(mg/kg
powder)
(%)
Level
Conc.
(ng/mL)
Conc.
(ng/mL)
1849a
CLC-10b
1.99
0.1445
6.276
2.172
109
Blank
0
8.5 – 60
0.1532
6.981
2.278
114
1
4.981
0.2006
8.454
2.107
106
2
9.962
1.0020
40.21
2.006
101
3
19.92
1.0006
41.05
2.051
103
4
39.85
1.5177
58.69
1.934
97.2
5
59.77
1.5082
57.60
1.910
96.0
6
79.70
2.0006
69.39
1.734
87.1
7
99.62
2.0003
69.28
1.732
87.0
Table 4. A representative calibration standard data.
Level
Concentration
(ng/mL)
HPLC Peak area
Amount
(ng/mL)
Error
1
5
0.2019
6.21
24%
2
10
0.6600
9.00
10%
3
20
2.2932
18.61
7%
4
40
6.1743
41.03
3%
5
60
9.2305
60.45
1%
6
80
11.353
77.66
3%
7
100
12.863
103.9
4%
Figure 1. A representative calibration curve.
Bio-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE