425 / 670
104.1% for acetylcarnitine. The level of detection (LOD) was determined by the mean baseline
noise + (3 x standard deviation) from 10 blank replicates, and then adjusted with the default
weights and dilutions used in the method. An LOD of 0.0034 mg/100g for both free and total
carnitine analyses, and an LOD of 0.0047 mg/100g for both free and total choline analyses were
achieved. An LOQ of 0.05 mg/100g was obtained for both free and total choline and carnitine.
The LOQ was calculated from the lowest working standard concentration through the default
weights and dilutions used in the method. The analytical range of the method is from the stated
LOQ to 250 mg/100g choline and 20 mg/100g carnitine, and is supported by the data collected
from the precision and accuracy experiments. The method meets all requirements of SMPR
2012.010 and 2012.013 for L-carnitine and total choline. The only exception is the inability to
distinguish between L- and D-carnitine with this method.
References
(1)
AOAC SMPR 2012.010 (2013)
J.AOAC Int.
96
, 488.
(2)
AOAC SMPR 2012.013 (2013)
J.AOAC Int.
96
, 492.
Chol-08/Carn-07 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE