450 / 670
Table 27. NIST SRM 1849a, Total Carnitine n = 12
Calculated Amount
Mean
SD
(mg/100g)
(mg/100g)
(mg/100g)
%RSD
r
14.8
15.3
0.44
2.9
15.6
15.5
16.0
15.4
0.81
5.3
15.8
14.5
15.0
15.0
0.10
0.7
14.9
15.1
15.7
15.4
0.26
1.7
15.2
15.3
Mean (mg/100g)
Certified Mass Fraction Mean / Range (mg/100g)
%RSD
r
15.3
NA
3.2
Recommendations
It is the recommendation of the Study Director the method is fit-for-purpose in
determining total choline and L-carnitine in infant formula and adult/pediatric nutritional
formula and that it be adopted as AOAC First Action
Official Method
status.
Acknowledgements
The Study Director would like to thank Jeff Shippar who developed the LC/MS/MS
conditions for this method. The Study Director would also like to thank Justin Gilmore
who was a major part in completing the SLV and contributing modifications to make a
more efficient and rugged method overall. Thank you to Katherine Nass, Tom Shook,
and Brian Smith who contributed to the collection of data over the course of the SLV.
Chol-08/Carn-07 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE