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39

Appendix A

Examples from Qualitative Methodology

1.

Figures A-4a and A-4b show an example of product #5 (SPIFAN matrix Placebo Child Formula Powder). As shown

in Figure A-4b there are no significant peaks after 36 minutes as is shown in Synergy 1 or HP therefore it must belong

to the lowest average DP range.

2.

Figure A-5 shows powdered milk based adult powder containing Synergy 1. This can be seen by the presence of both

GF

3

/GF

4

as well as peaks after 36 minutes.

3.

Figure A-6 shows a powdered infant formula containing GOS that was spiked with HP. This can be confirmed by the

absence of GF

3

/GF

4

as well as peaks after 36 minutes.

4.

Figures A-7 through A-14 are examples of products that do not contain fructan material.

a.

Products containing maltodextrin with a high degree of polymerization may be difficult to differentiate from

inulin containing products. For example the product in A-9 contains maltodextrin (blue trace), but no fructan

material. It is overlaid with the HP standard (black trace) to demonstrate how they look different. Note how the

blue product trace containing maltodextrin stops short of the wash while the HP standard keeps going. One

must be vigilant to avoid a false identification in a case like this.

b.

Unsurprisingly other carbohydrate commodities will have varying profiles as well (figures A-21 through A-24).

Figure A-1

scFOS STD

Figure A-2

Synergy 1 STD

Fos-04 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE