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Analysis of Vitamin D

2

and Vitamin D

3

by LC-MS/MS in Milk

Powders, Infant Formulas, and Adult Nutritionals

Page 10 of 10

PA

SILD3

= peak area of SIL d6-vitamin D

3

in sample (

units

: none)

SILIS

conc

= concentration of SIL d6-vitamin D

3

in

SILIS

(

units

: ng mL

-1

)

L = slope of calibration curve (

units

: none)

SILIS

alqt

= volume of

SILIS

aliquot spiked to sample (

units

: mL); [0.5 mL]

1000 = mass conversion factor (

units

: μg g

-1

to mg g

-1

)

100 = mass conversion factor (

units

: mg g

-1

to mg hg

-1

)

S

mass

= mass of sample (

units

: g)

The concentration of vitamin D

2

or D

3

as IU hg

-1

in the sample is calculated as:

40x ) hgg(

Result

) hg (IU

Result

-1

-1

P

where:

40 = dietary conversion factor (

units

: μg hg

-1

to IU hg

-1

)

Report:

Report result as μg hg

-1

to 1 decimal place or IU hg

-1

to 0 decimal places.

VitD-18 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE