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AsureQuality Laboratory Services
Table 4: Repeatability
Average
CLC10-b
197.42 201.01 196.08 196.28 197.93 201.54 198.54 198.39 199.34 198.81 197.00 197.30 198.30 0.86
11750017V3 12.77 12.73 12.67 12.77 12.69 12.69 12.53 12.73 12.61 12.62 12.65 12.70 12.68 0.55
1172572116 34.68 34.50 34.20 35.24 35.17 35.13 36.05 35.09 36.19 36.97 35.19 35.02 35.29 2.19
117257651Z 39.76 39.99 39.98 39.16 40.12 40.50 39.37 40.77 40.87 40.96 39.50 40.70 40.14 1.54
00394RF00 248.67 249.85 254.88 237.61 258.75 261.19 256.65 259.83 255.74 259.20 254.28 256.30 254.41 2.55
00412RF00 91.08 90.66 88.99 89.01 89.70 91.71 89.92 90.81 89.56 90.20 90.69 90.72 90.25 0.93
00403RF00 69.17 69.30 70.41 69.88 71.88 72.28 71.35 71.45 72.14 71.83 70.52 71.03 70.94 1.52
D04HTCVV 35.48 35.24 34.23 33.92 33.36 34.28 33.01 33.17 32.94 32.96 31.71 32.94 33.60 3.19
E29JVLV
43.30 43.25 45.40 44.72 45.02 44.38 45.29 44.85 45.15 44.78 44.76 45.08 44.67 1.58
Control Milk F 5.45 5.47 5.36 5.34 5.40 5.44 5.38 5.43 5.20 5.31 5.15 5.37 5.36 1.83
00414RF00 54.02 55.42 55.50 55.16 52.36 52.07 53.67 53.78 53.23 53.91 55.48 54.21 54.07 2.16
00406RF00 68.88 65.17 63.77 64.85 66.78 67.48 65.51 66.54 65.64 65.36 63.92 62.13 65.50 2.74
SRM 1849a
198.46 0.63
Biotin (μg/100g)
RSD
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
199.97
199.65
196.50
197.87
198.37
198.42
SPIFAN SLV
Samples
5.7 Comparison with alternative immunoaffinity column
The laboratory has analysed the SPIFAN SLV samples using immunoaffinity column
manufactured by Biotez Berlin Buch GmbH (Product, BioTeZ IAC Biotin) and compared with
the average biotin results obtained from Biopharm Rhone, EASI-EXTRACT biotin column.
The 3 RTF samples from the SPIFAN kit were not included in the comparison study due to
the microbial growth observed in the milk samples (Table 5).
Table 5: Comparison with alternative immunoaffinity column
SPIFAN SLV Samples
Biotin (μg/100g)
R- Biopharm
Biotez
Difference
CLC10-b
198.30
199.87
1.57
11750017V3
12.68
12.85
0.17
1172572116
35.29
36.04
0.75
117257651Z
40.14
41.29
1.15
00394RF00
254.41
243.68
10.73
00412RF00
90.25
86.62
3.63
00403RF00
70.94
69.24
1.70
D04HTCVV
33.60
30.57
3.03
E29JVLV
44.67
43.13
1.54
SRM 1849a
198.46
199.99
1.53
Bio-02 (February 2016)
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