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Mechanobiology of Disease
Poster Abstracts
100
3-POS
Board 3
Comprehensive Analyses of Gene Expression Patterns in Early Mouse Early Embryos
Experiencing Chemical and Mechanical Stimuli
Yuka Asano
1,2
, Koji Matsuura
1,2
.Keiji Naruse
2
.
2
Okayama University, Okayama, Japan.
1
Okayama University of Science, Okayama, Japan,
In vitro
mammalian embryonic development can be enhanced by mimicking physiological
conditions, which can provide better infertility treatment. By comprehensive analyses of gene
expression, we evaluated differences in gene expression patterns of ICR mouse blastocysts
cultured
in vitro
from two-cell embryo stage using different media under a static or dynamic
culture system to subject the embryos to mechanical stimuli (MS). To compare the influences of
the medium on early mouse embryonic development, we used mW medium (mW-S) and KSOM
medium (KSOM-S) under static culture. The influences of MS in mW medium were investigated
using the tilting embryo culture system (TECS), which improves blastocyst development. We
compared the gene expression patterns between the TECS (mW-T) and mW-S groups.
Blastocyst development rates in the mW-S, KSOM-S, and mW-T groups were 62.6%, 94.1%,
and 70.8%, respectively (P < 0.01). We extracted total RNAs thrice from the three groups, and
the total number of blastocysts was approximately 80 × 9 = 720. RNA amplification, RNA-Seq
library preparation, and RNA sequencing were consigned to Hokkaido System Science Co. Ltd.
Upon comparison of the relative gene expression levels between the KSOM-S and mW-S
groups, the gene expression level in the trophectoderm (TE), which functions as a source of
embryonic nutrition, was improved in culture conditions under KSOM medium compared with
that in culture conditions under mW medium.
Tagln2
gene expression as a TE marker increased
(P < 0.01) under KSOM culture.
Gdf3
gene expression levels related to epiblast (EPI)
development in the mW-T group was higher than that in the mW-S group (P < 0.01). MS
improved EPI development under the same medium conditions. The expression patterns of EPI
and TE markers differed between the culture systems with chemical stimuli by medium and MS.
We consider that optimized medium components can improve both TE and EPI development and
that MS can improve the gene expression of EPI markers. However, the gene expression level
induced by MS would be relatively lower in TE than in EPI.