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Mechanobiology of Disease
Poster Abstracts
124
78-POS
Board 78
Curvature Effect on Monolayer Cell Migration
Wang Xi
1,2
, Surabhi Sonam
2,3
, Benoit Ladoux
2,4
, Chwee Teck Lim
1,2,5
,
1
Centre for Advanced 2D Materials, Singapore, Singapore,
2
Mechanobiology Institute,
Singapore, Singapore,
3
Department of Biomedical Engineering and Department of Mechanical
Engineering, Singapore, Singapore,
4
Institut Jacques Monod, Paris, France,
5
Singapore MIT
Alliance Research and Technology, Singapore, Singapore.
In vivo cell sheets constitute a variety of curved architectures such as lung alveoli, kidney
nephron and breast acini. Notably, during certain organ development and wound healing
processes, cell monolayers often advanced on a curved surface. While a considerable amount of
effort has been made to study monolayer cell migration, most of the studies have primarily used
2D-cell culture systems, which fail to recapitulate the complex geometric nature of tissues and
organs. The mechanistic basis of cell behavior on a 3D curvature also remains poorly
understood. Here, we investigate monolayer cell migration on various curved surfaces. We study
intra-cellular arrangements, cell polarity and cell sheet velocity. Our findings reveal interesting
insights into migration of cell monolayer in response to extent of curvature and can assist in
better understanding tissue morphogenesis.