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Mechanobiology of Disease
Poster Abstracts
117
54-POS
Board 54
Magnetic Control of the Gating of Bacterial Mechanosensitive Channel Mscl for Smart
Liposomes
Yoshitaka Nakayama
1
, Mislav Mustapic
2,3
, Haleh Ebrahimian
1
, Pawel Wagner
2
, Jung H. Kim
2
,
Md Shahriar Hossain
2
, Josip Horvat
2
, Boris Martinac
1,4
.
1
Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia,
2
University of
Wollongong, North Wollongong, NSW, Australia,
4
University of New South Wales,
Darlinghurst, NSW, Australia.
3
University of Osijek, Osijek, Croatia,
Liposomal drug delivery systems (LDDSs) are effective tools used for the treatment of diseases
especially when highly toxic pharmacological agents are utilized. In order to regulate the drug
release from liposomal capsules, remotely controlled nanovalves with a large conductive pore
are required. The bacterial mechanosensitive channel of large conductance, MscL, presents an
excellent candidate biomolecule for the LDDSs. In this study, we developed superparamagnetic
nanoparticles for the activation of the MscL nanovalves by magnetic field. Synthesised
CoFe
2
O
4
nanoparticles with the radius of less than 10 nm were labelled by SH-reactive chemical
groups for attachment to M42C MscL mutant. Activation of MscL by magnetic field with the
nanoparticles attached was examined by the patch clamp technique showing that the number of
activated channels under ramp pressure increased upon application of the magnetic field. In
parallel, we have tested and have not observed any cytotoxicity of the nanoparticles in human
cultured cells. Our study suggests the possibility of using magnetic nanoparticles as a specific
trigger for activation of MscL nanovalves for drug release in LDDSs.