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Mechanobiology of Disease
Poster Abstracts
54
31-POS
Board 31
Young and Senescence Fibroblast Cells Are Different in Mechanical Properties!
Samira Khalaji
1
, Fenneke KleinJan
1
, Vida Farsam
2
, Karin Scharffetter-Kochanek
2
, Kay-E
Gottschalk
1
.
1
Institute of Experimental Physics, Ulm University, Ulm, Germany,
2
Klinik für Dermatologie
und Allergologie, Ulm University, Ulm, Germany.
Biological aging is a complex, multi-dimensional process that makes physiological changes
taking place over a long period of time. This process takes up with macroscopic and microscopic
scale alterations within individual cells, over transformations in tissues and organs and to
changes of the whole organism. Cellular senescence is already characterised based on age
associated molecular markers in gene and protein pattern expression such as telomere
dysfunction. But there is little information regarding relationship of age and the mechanical
properties of cells. Biomechanical properties are vital properties for the cells and tissues of living
organisms. Estimation of the mechanical properties of a cell depends on a method of
measurement, theoretical model as well as cell state. Hence, we employed a variety of methods
such as passive microrheology and atomic force microscopy on primary dermal fibroblasts from
male human with CPD 17-35 (young) and CPD 58-59 (senescence) as a model of in vitro
replicative senescence. Our primary result shows significant differences in the viscoelastic
properties of young and senescence fibroblasts depending on in vitro senescence level and also
compared to older studies.