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Analytical Challenge
•
Traces of latex containing aloin may be present in inner
leaf juice production, or substantial amounts (several
percent) are found in leaf juice prior to removal, usually
via activated carbon filter
•
Very low concentration (ppb/ppt levels) in purified aloe
vera juice and dry juice ingredients and finished products
R l t d
d / th i
l t
d
•
e a e compoun s an ocyan ns can co‐e u e an can
lead to misinterpretation
•
Need cost effective test method to find trace levels
(ppb/ppt)
Current analytical methods and techniques
Currently four analytical techniques are available for aloin
testing:
•
Thin Layer Chromatography: For identification TLC does
not provide quantitative results for low levels (ppb/ppt)
•
HPLC: Good resolution, cost effective, easy sample
preparation, most labs can run HPLC
•
LC‐MC and GC‐MS: good resolution, expensive
instrumentation and maintenance as compared to
LC/HPLC, need trained chemist for interpretation