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Challenges

Chemical differentiation of oolong tea and

black tea/orange pekoe tea

Variation of tea catechins content due to

Different geographic locations

Seasonal changes

Different processing methods of tea products

Different extraction methods

Different solvents

Interference from other ingredients.

Existing Methods – General Literatures

HPLC (UPLC) UV‐MS: Identification and quantitation 

catechins, caffeine, flavonoids, phenolics, amino acids

HPTLC: Identification 

NMR: Metabolic Profiling

FTIR and NIR: Identification, Differentiation 

GC: Aroma and flavor

UV: Total polyphenols