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STAKEHOLDER PANEL ON Dietary 

Supplements

Background & Fitness for Purpose

Vitamin D

John Austad

d

AOAC 2015 Mi ‐Year Meeting

Rockville, MD

19 March 2014

Background on analyte

Vitamin D 

Two Primary Forms:

Ergocalciferol (Vitamin D

2

)

Formed from UV‐Light Irradiated Ergosterol

C

28

H

44

O  Molecular Mass = 396.55 g/mol

Ch l

l if l (Vit i D )

o eca c ero am n 

3

Formed from UV‐Light Irradiated 7‐dehydrocholesterol

C

27

H

44

O  Molecular Mass = 384.64 g/mol