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Significance
•
Promotes calcium absorption
•
Essential for bone growth
•
Prevents Rickets in Children
•
Prevents osteomalacia in adults
C bi
i h C l i
d l f
•
om ne w t a c um, protects a u ts rom
osteoporosis
General Analytical Needs
•
Method should
–
Separate and accurately determine both Vitamin D
2
and D
3
–
Determine Pre Vitamin D
2
and D
3
–
Determine 25 Hydroxy D
2
and D
3
–
Also determine the above in raw materials used to
produce/formulate dietary supplements