AOAC SMPR 2014.XXX; Version 2; March 19, 2014
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Method Name: Determination of Amino Acids in Infant and
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Adult/Pediatric Nutritional Formula
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Approved by:
Stakeholder Panel for
Infant Formula and Adult Nutritionals
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Final version date
:
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Effective date:
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Intended Use:
Reference method for dispute resolution.
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1.
Applicability:
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Determination of free and/or total proteinogenic L-
α
-amino acids and taurine (as
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shown in Table 1) in all forms of infant, adult, and/or pediatric formulas (powders,
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ready-to-feed liquids, and liquid concentrates).
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2.
Analytical Technique:
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Any analytical technique that meets the following method performance
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requirements is acceptable.
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3.
Definitions:
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Accuracy
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The closeness of agreement between the average of an infinite number of replicate
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measured quantity values and a reference quantity value.
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Adult/Pediatric Formula
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Nutritionally complete, specially formulated food, consumed in liquid form, which may
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constitute the sole source of nourishment
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, made from any combination of milk, soy,
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rice, whey, hydrolyzed protein, starch, and amino acids, with and without intact
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protein.
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Infant formula
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Breast-milk substitute specially manufactured to satisfy, by itself, the nutritional
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requirements of infants during the first months of life up to the introduction of
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appropriate complementary feeding
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,
made from any combination of milk, soy, rice,
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whey, hydrolyzed protein, starch, and amino acids, with and without intact protein.
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Limit of Detection (LOD)
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The minimum concentration or mass of analyte that can be detected in a given matrix
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with no greater than 5% false positive risk and 5% false negative risk.
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Limit of Quantitation (LOQ)
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The minimum concentration or mass of analyte in a given matrix that can be reported
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as a quantitative result
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Repeatability
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Variation arising when all efforts are made to keep conditions constant by using
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the same instrument and operator, and repeating during a short time period.
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Expressed as the repeatability standard deviation (SD
r
); or % repeatability
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relative standard deviation (%RSD
r
).
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Corresponds to the VIM definition for “trueness”.
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AOAC Stakeholders Panel for Infant Formula and Adult Nutritionals (SPIFAN); 2010.
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Codex Standard 72
–
1981.
