AOAC ISPAM Meeting eBook, March 17 2015

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PROTOCOL • Elution
• Concentration
CEN/TC275/WG6/TAG4
ISO/TS 15216
PART 1 & 2
Virus : detection method?
QUANTITY
PURIFIED
VIRAL RNA
From a know volume of
concentrate/extraction by standard
method
100
cm2 25g 2g 1L
5
Qualitative Quantitative
ANALYSIS
2 PARTS
Real-time (TaqMan) RT-PCR
Determination
Virus : detection method?
CEN/TC275/WG6/TAG4
ISO/TS 15216
Part 1 & 2
PRETREATMENT & EXTRACTION OF
NUCLEIC ACIDS
SWABBING FOLLOWED BY ELUTION 100c
m 2
25g ELUTION WITH AGITATION & PRECIPITATION
WITH PEG / NaCl
6
2g
1L
DIGESTIVE TISSUE PRETREATMENT BY
CRUSHING AND PROTEINASE K
ADSORPTION & ELUTION ON LOADED MEMBRANES &
CONCENTRATION / ULTRAFILTRATION