67 / 120
CEN/TC275/WG6/TAG
4
ISO/TS 15216
OPTIMIZED FOR
ROUTINE ANALYSIS
Virus : detection method?
FAST
ROBUST
SENSITIVE
SPECIFIC
% AMPLIFICATION
EFFICACY
95
MULTI-
PLATEFORMS
11
STANDARDIZED
AMPLIFICATION
S
A
MULTIPLEXING
M
EXTERNAL CONTROL
MENGOVIRUS
MULTI-MATRICES
CEN/TC275/WG6/TAG4
ISO/TS 15216
VALIDATED PROCEDURE DIAGRAM
Virus : detection method?
SWABBING FOLLOWED
BY ELUTION
100
cm
2
25g
GLYCINE BUFFER ELUTION
PRECIPITATION PEG / NaCl
CLARIFICATION
CHLOROFORM BUTANOL
HARD
SURFA
CES
SALADS
VEGGIES
FRUITS
HERBS
SPICES
Nucleic acids
extraction on
1mL
concentrated
virus, lysis with
guanidine
isothiocyanate
Mengovirus
DETECTION
VIRUS PRETREATMENT
MENGOVIRUS ADDED
EXTRACTION RNA
DETECTION
12
2g
1L
DIGESTIVE TISSUES
ELUTION PROTEINASE K
INCUBATION 37
°
THEN
60
°
CENTRIFUGATION
CONCENTRATION /
FILTRATION TANGENTIAL
CASSETTES, PRECIPITATION
PEG CLARIFICATION
CHLOROFORM BUTANOL
BIVALVE
MOLLUSKS
LIQUID
SAMPLES
& silica binding.
Elution RNA in
100 µl
(= MiniMag
equipment +
Nuclisens
reagents
bioMérieux)
& targets
RESULTS
INTERPRET
ATION