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Question
Response
General
AOAC Method Number:
CHON-004
Method Title:
Determination of Chondroitin Sulfate Content in Raw
Materials and Dietary Supplements by High-Performance
Liquid Chromatography with Ultraviolet Detection After
Enzymatic Hydrolysis
Method Author(s):
David Ji, Mark Roman, Joseph Zhou, and Jana Hildreth
Reviewer Name:
Darryl Sullivan
Summary of Method
This method measures chondroitin sulfate after hydrolysis
with chondroitinase ACII enzyme that form un-, mono-, di-,
and trisulfated unsaturated disaccharides. The resulting
disaccharides are quantified using ion-pairing liquid
chromatography with UV detection at 240 nm. The quantities
of the individual disaccharides are summed to yield the total
amount of chondroitin sulfate in the test samples.
Method Scope / Applicability:
This method is applicable to the determination of chondroitin
sulfate in raw materials, and finished products. It has been
studied on capsules, dietary supplement tablets, softgels, and
liquids.
General Comments About the
Method:
The method has been through a single laboratory validation
and has been well studied.
Method Clarity
This method is clearly written, and easy to follow and
understand.
Pros/Strengths
This method offers a way to measure chondroitin sulfate
through the quantification of the component disaccharides.
The method has been exposed to a significant amount of
validation. The published method performance for
repeatability precision, and accuracy are good.
Cons/Weaknesses
This method utilizes a very specific enzyme and requires the
use of some unusual disaccharide standards.
Supporting Data
General Comments
This method has been used successfully and demonstrates
CHON-004