40 / 116
Supporting Data
General Comments
Not given
Method Optimization
No
Performance Characteristics
Analytical Range:
Not Given
LOQ:
Not Given
Accuracy/Recovery
102.5-103.9 %
Precision (RSDr)
2.38 %
Reproducibility (RSDR)
1.74 %
System Suitability
May be suitable for routine analysis
Safety Review
Is the method safe to use as
written?
Yes
Are there any safety concerns
with any component, instrument,
reagent or instruction of the
method? If yes, please clearly
state the concerns.
No
Does the method include all
necessary safety precautions
and/or warning statements? Yes
or No. If No, please suggest
potential precautions or warnings
that can be added to the method
for users or recommend safer
option(s) if warranted.
Yes
Recommendations
Final Recommendation:
Several spectrophotometric assays for sulfated GAG
quantification in biological samples based on the use of
DMMB have been reported. All of these methods rely on the
absorption at about 525 nm of the GAGs-DMMB complex
formed after addition of the dye to biological samples. In all
reported methods, this complex is formed in a reaction
mixture containing a large excess of the dye, which absorbs
in wavelengths that might interfere with the detection of
the GAG-dye complex. Among other drawbacks of DMMB
classical methods, the presence of a precipitate of the GAG-
dye complex has been reported to render quantification
CHON-003