SPDS Set 1 ERP Book

Question

Response

General

AOAC Method Number:

CHON-003

Method Title:

Chondroitin by IR and Dimethylene Blue (DMBB)

spectrophotometry

Method Author(s):

Not stated

Reviewer Name:

Aniko Solyom

Summary of Method

Colorimetric method for the quantification of chondroitin

sulfate.

Method Scope / Applicability:

According to the method description, the method is

applicable for the quantification of chondroitin sulfate in

raw materials and finished products, such as tablets.

General Comments About the

Method:

Method Clarity

Detailed SOP style

Pros/Strengths

Relatively quick and cheap method for the analysis of raw

materials and probably simple finished products.

Cons/Weaknesses

1. Chondroitin-DMMB complex will aggregate immediately,

therefore the reading time should be the same for each

sample/standard. Room for significant human error!

2. Not a specific method for complex samples. If anything

(including adulterants)in the sample reacts with DMMB and

absorbs at 525 nm, the results are not valid.

Supporting Data

General Comments

Method Optimization

Not Provided

Performance Characteristics

Analytical Range:

Calibration curve: 5-15 microgram chondroitin sulfate (only

3 point calibration curve)

Chondroitin sulfate content of the

CHON-003