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CHON-002
Question
Response
General
AOAC Method Number:
CHON-002
Method Title:
Isotachophoretic Determination of GA and CS in Dietary
Supplements
Method Author(s):
Vaclavikova, E., et al.
Reviewer Name:
Curtis Phinney
Summary of Method
This method is based on (cITP), a capillary electrophoretic
technique based on ionic mobility, with conductivity and UV
(254 nM) detection.
Method Scope / Applicability:
Applicable to dietary supplements and raw materials.
General Comments About the
Method:
The technique is very well described in two related papers.
The authors investigated the role of economically significant
and common impurities in great detail.
Method Clarity
The method is described fully, with considerable background
and supporting information.
Pros/Strengths
Rigorous experimental design
Addresses significant quality concerns
Addresses economic adulterants
Simple sample prep and reagents
Cons/Weaknesses
Validated using alternate guidelines (ICH '95, '96)
No explicit system suitability step
No inter lab data, 2-day inter day study
Supporting Data
General Comments
Extensive supporting data and discussion of interferences.
Supporting data may not address all SMPR
Method Optimization
Exhaustively optimized method. Raw data is not presented
in this paper.
Performance Characteristics
Analytical Range:
< 1% to 100%