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PDE5-004
would be needed as well.
Supporting Data
General Comments
Multiple dietary supplement matrices need to be
evaluated to provide an indication of the false
positive/false negative rate. However, given this method
measures activity and not concentration, it is a good
indicator of the potency of the inhibitor, whereas
molecular identification of PDE5 inhibitors that have
been altered to escape detection would not provide.
Method Optimization
Not described
Performance Characteristics
Analytical Range:
Not described. However, indicated between 2.5 and 25
mg or a pharmacological dose. Concentrations are
relative to the IC50 (inhibition concentration) relative to
known PDE5 inhibitors (e.g. sildenafil, tadalafil, etc.)
LOQ:
Accuracy/Recovery
Not described
Precision (RSDr)
Not described
Reproducibility (RSDR)
asdas
System Suitability
Safety Review
Is the method safe to use as written?
Safety for handling enzymes and solutions was not
described
Are there any safety concerns with
any component, instrument, reagent
or instruction of the method? If yes,
please clearly state the concerns.
There should be statement of safety or precautionary
measures when handling reagents and there are none.
Does the method include all
necessary safety precautions and/or
warning statements? Yes or No. If
No, please suggest potential
precautions or warnings that can be
added to the method for users or
recommend safer option(s) if
warranted.
No. Handling of reagents and enzymes need to have
some statement of safety and handling procedure
requirements.
Recommendations