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PDE5-004

Question

Response

General

AOAC Method Number:

PDE5-004

Method Title:

Testing for Phosphodiesterase Type 5 (PDE5) Inhibitors in

Dietary Supplements

Method Author(s):

Not stated

Reviewer Name:

Teresa Cain

Summary of Method

This is bioluminescent assay method to screen for presence

of PDE5 inhibition in a test sample based on a commercially

available assay kit which is sensitive and reliable.

Method Scope / Applicability:

Scope of method is to screen for PDE5 inhibiting

compounds as adulterants in dietary supplements.

General Comments About the

Method:

This method shows value in being a quick and easy

screening method to detect the presence of drug

adulterants in dietary supplements.

Method Clarity

Method uses a commercially available Assay Kit with clear

instructions.

Pros/Strengths

*Method is simple and easily applied as a rapid screening

method for the presence of compounds with PDE5

inhibition.

*Sensitivity of the method allows dilution of possible

interfering matrix components.

*False positives are extremely unlikely due to dilution of

the sample matrix.

*Method states that assay may be adaptable to a hand-

held device.

Cons/Weaknesses

Non-specific method without capability of identifying

analyte present

Supporting Data

General Comments

NA

Method Optimization

NA