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PDE5-005
Question
Response
General
AOAC Method Number:
PDE5-005
Method Title:
Single Laboratory Validation Study of a Method for
Screening and
Method Author(s):
Lukas Vaclavik1, John R. Schmitz2, Jean-Francois
Halbardier1 and Katerina Mastovska2*
Reviewer Name:
Teresa Cain
Summary of Method
Liquid chromatography-high resolution mass
spectrometry (LC-HRMS) method for
screening,identification and quantitation of PDE5
inhibitors in dietary ingredients and supplements.
Method Scope / Applicability:
Screening, Identification, Quantitation of adulterants in
dietary supplements
General Comments About the
Method:
Single laboratory validation performed.
Method Clarity
Method is comprehensive and detailed and can be clearly
followed given equivalent instrumentation.
Pros/Strengths
*Appropriate for screening, identification and
quantitation of adulterants.
*Easily adaptable to new analogues.
Cons/Weaknesses
*Fragmentation for the sildenafil class of drugs lacks
structurally significant fragment ions that are typically
seen in literature above m/z 283 or 299.
*Instrumentation utilized is expensive and requires
technically trained operators.
Supporting Data
General Comments
See other fields.
Method Optimization
Since some of the analytes show structurally significant
fragments when there is some parent ion visible, it is
recommended to adjust the fragmentation settings to
less energetic settings, particularly for the sildenafil class