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. 97, N
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. 5, 2014
both 0.09 µg/mL, and the LOQs were 0.23 and 0.21 µg/mL,
respectively (Table 2).
Precision
From the nine test materials used in this study, five test
materials (TM2, TM3, TM5, TM6, and TM8) were above the
LOQ and had a HorRat range of 0.3 to 2.0 and RSDr values of
0.61–8.64% as summarized in Table 3. Three of the test materials
(TM1, TM7, and TM9) were below the LOQ but were detected,
and the one test material (TM4) was below the LOD; therefore,
no HorRat and RSD
r
values were reported for these materials.
Accuracy
The average recovery range of aloin A and aloin B in both
solid and liquid matrixes was 84.41–108.86% with an RSD
r
of
0.15–8.64%. Tables 4a and b contain the summarized results for
each individual aloin at the three different concentration levels.
Stability of Mixed Standard
The stability of the aloins in reference standards, as well as
test material solutions, was found to be higher with the addition
of an acid. It was determined during method optimization that
0.1% acetic acid was adequate to provide good peak shape and
retain analyte stability over a period of time.
Storage of the aloin reference standards with the addition of
an acid provided consistent peak area after 48 h when exposed
to room temperature conditions with an average difference of
2–3% mAu*s. In the nonacidified solutions, the aloins started
to degrade after 8 h at room temperature, and with an average
difference of 23–28% mAu*s after 48 h from the initial time
point.
Conclusions
This method for the determination of aloin A and aloin B in
raw materials and select finished products containing
A.
vera
leaf juice was subjected to an SLV study according to AOAC
INTERNATIONAL guidelines. All parameters investigated
in the validation study were in compliance with the AOAC
INTERNATIONAL guidelines. The described method is thus
considered to be suitable for its purpose and ready for further
validation studies.
Acknowledgments
We would like to acknowledge Aloecorp (Ontario, CA),
International Aloe Science Council (Silver Spring, MD) and
Mark Roman from Tampa BayAnalytical Research (Tampa Bay,
FL) for providing the materials for this study. In addition, we
would like to acknowledge the ChromaDex for supplying the
reference standards used in the validation study.
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