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Aloe SLV Method Supplementary Information
Precision: The precision (RSDr) reported in the publication is intermediate precision (different days,
calibration curves, solutions, etc); which is more rigorous in comparison to AOACs guidelines for
repeatability as specified in the SMPR. Threfore, we have complied the repeatability according to AOAC
guidelines below. The repeatability (%RSDr) between replicate samples on the same day for day 1 of the
analysis (4 replicate samples) is summarized below for the samples which contained Aloins above the
quantitation limit.
Repeatability (%RSD)
Aloin A
Aloin B
TM2
3.6
2.8
TM3
0.83
4.3
TM5
1.4
2.1
TM6
0.83
0.74
TM8
0.85
0.51
LOD/LOQ concentration in Samples: The LOD/LOQ reported in the publication is concentration of the
calibration solution. The back calculation for liquid and dry matrices are summarized below:
Liquid samples (as per manuscript):
LOD
aloin A &B: 0.09
µ
g/mL;
LOQ
Aloin A: 0.23
µ
g/mL, Aloin B: 0.21
µ
g/mL.
Powders & capsules:
LOD
Aloin A & B: 0.9
µ
g/g;
LOQ
Aloin A: 2.3
µ
g/g, Aloin B: 2.1
µ
g/g