AOAC RI ERP E-Book - DS DF
Figure 2017.xxE. Chromatograms on TSK-Gel G2500PWXL columns of glucose/glycerol mixtures. A mixture of glycerol (100 mg) and glucose (100 mg) was analysed according to the RINTDF procedure. The ethanolic filtrate (for SDFS determination) was concentrated to dryness and re-dissolved in 32 mL of deionised water. A sample of this was analysed by HPLC; a) directly with no desalting and no columns, the TSKgel ® PW XL guard column and a Bio-Rad ® de- ashing pre- cartridge containing cation and anion deionizing cartridges in place; b) a sample (5 mL) was desalted by mixing with 1.5 g of Amberlite ® FPA53 (OH − ) and 1.5 g of Ambersep ® 200 (H + ) resins over 5 min and the supernatant was analysed by HPLC with no Bio-Rad ® de-ashing pre-cartridges in place; c) Sample b) was analysed with a Bio-Rad ® de-ashing pre-cartridges in place. Desalting with resins in a polypropylene tube, as described here, removes > 95% of the salt from the sample, thus ensuring more efficient use of the expensive Bio-Rad ® de-ashing pre-cartridges. This desalting step increases the effectiveness of the de-ashing cartridges and allows up to 10-times more samples to be chromatographed before the need to regenerate or replace the de-ashing cartridges. . AOAC Research Institute ERP Use Only
Method updated 2008--08-10 This copy printed 22 August 2017
30
Made with FlippingBook HTML5