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Single Lab Validation Report for GOS in Infant Formula and Adult Nutritionals

7

Figure 2: Linear calibration curve for lactose

Although the R

2

values were acceptable, the residue plots of the back-calculated concentrations showed

parabolic trending on the linear fit (Figure 3 and 4).

Figure 3: Residue plot of the deviation from the actual concentration by linear calibration for galactose

y = 0.0696x + 0.0003

R² = 0.9994

0

0.2

0.4

0.6

0.8

1

1.2

1.4

0.00

5.00

10.00

15.00

20.00

Ratio Area/Area IS

Lactose Concentration (µg/mL)

Linear Calibration Curve Lactose

-10

-5

0

5

10

15

20

25

0.00

5.00

10.00

15.00

20.00

% deviation from actual concentration

Galactose Concentration (µg/mL)

Residu Plot Galactose Linear

GOS-02 SLV

FOR ERP USE ONLY

DO NOT DISTRIBUTE