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Single Lab Validation Report for GOS in Infant Formula and Adult Nutritionals

8

Figure 4: Residue plot of the deviation from the actual concentration by linear calibration for lactose

Therefore, a quadratic fit was applied for both components (Figure 5 and 6).

Figure 5: Quadratic calibration curve for galactose

-6

-4

-2

0

2

4

6

8

10

12

14

16

0.00

5.00

10.00

15.00

20.00

% deviation from actual concentration

Lactose Concentration (µg/mL)

Residue Plot Lactose Linear

y = -0.0004x

2

+ 0.1018x + 0.0034

R² = 1

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

2

0.00

5.00

10.00

15.00

20.00

Ratio Area/Area IS

Galactose Concentration (µg/mL)

Quadratic Calibration Curve Galactose

GOS-02 SLV

FOR ERP USE ONLY

DO NOT DISTRIBUTE