SPIFAN Nutrients ERP Book 9-29-15

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Single Lab Validation Report for GOS in Infant Formula and Adult Nutritionals
9
Figure 6: Quadratic calibration curve for lactose
The residue plots of the back-calculated concentrations on the quadratic fit showed a deviation of less
than 1.5% for the galactose (Figure 7) and of less than 8% for the lactose (Figure 8) without parabolic
trending. Therefore, calculations for precision and accuracy are based on quadratic fit.
Figure 7: Residue plot of the deviation from the actual concentration by quadratic calibration for
galactose
y = -0.0001x2 + 0.0716x - 0.0038
R² = 0.9995
0
0.2
0.4
0.6
0.8
1
1.2
1.4
0.00 5.00 10.00 15.00 20.00
Ratio Area/Area IS
Lactose Concentration (µg/mL)
Quadratic Calibration Curve Lactose
-1.5
-1
-0.5
0
0.5
1
0.00 5.00 10.00 15.00 20.00
% deviation from actual concentration
Galactose Concentration (µg/mL)
Residue Plot Galactose Quadratic
GOS-02 SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE