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Single Lab Validation Report for GOS in Infant Formula and Adult Nutritionals
17
6.
Method:
6.1.
GOS fractionation
•
Carbopac PA100 column plus guard
•
Eluent A = 1 M NaOH
•
Eluent B = 1 M NaAc
•
Eluent D = Milli-Q
•
Flow 1 mL/min
•
Column temperature 20°C
•
Tray temperature 10°C
•
Pressure upper limit = 4000 psi
•
Sample injection 25 µL
Table 5.3: Elution scheme for fractionation
Time (min) Eluent A (%) Eluent B (%) Eluent C (%) Eluent D (%) Curve
0.00
10
4
0
86
5
1.10
10
4
0
86
5
4.00
50
40
0
10
5
8.00
50
40
0
10
5
8.10
10
4
0
86
5
21.0
10
4
0
86
5
6.2.
GOS quantification
•
Carbopac PA1 column plus guard
•
Eluent A = 300 mM NaOH
•
Eluent B = 1 M NaAc
•
Eluent D = Milli-Q
•
Flow 1 mL/min
•
Column temperature 20°C
•
Tray temperature 10°C
•
Pressure upper limit = 3000 psi
•
Sample injection 25 µL
Table 5.4: Elution scheme for quantification
Time (min) Eluent A (%) Eluent B (%) Eluent C (%) Eluent D (%) Curve
0
5
0
0
95
5
20
5
0
0
95
5
33
32
0
0
68
5
35
32
0
0
68
5
39
32
50
0
18
5
43
32
50
0
18
5
45
32
0
0
68
5
48
5
0
0
95
5
63
5
0
0
95
5
GOS-02 SLV
FOR ERP USE ONLY
DO NOT DISTRIBUTE