SPIFAN Nutrients ERP Book 9-29-15

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Simultaneous Determination of Thiamine HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide),
Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant
Formula and Adult Nutritionals
CONFIDENTIAL METHOD FOR ERP ASSESSMENT ONLY
PAGE 2 OF 13
CHEMICALS & REAGENTS
Equivalents may be used unless otherwise specified. Standard and reagent preparations may be scaled
up or down as needed.
No. Reagent (specify grade,
if applicable)
Concentration Preparation
No.
Brand Shelf life Storage
1 Formic acid (CH2O 2), AR 98% N/A Merck as per COA Room
temperature
2 Hydrochloric Acid (HCl),
AR
37% N/A Merck as per COA Room
temperature
3 Hepafluorobutyric acid
(HFBA)
98% N/A Sigma as per COA Room
temperature
4 Methanol (MeOH;
CH 3OH), HPLC Grade
100% N/A J.T.
Baker
as per COA Room
temperature
5 Acetronitrile (ACN;
CH 3CN)
100% N/A J.T.
Baker
as per COA Room
temperature
6 Ammonium formate, AR ≥ 99% N/A Fluka as per COA 2-8 C
7 Ammonium hydroxide
(NH 4 OH), AR
28-30% N/A Merck as per COA Room
temperature
8 Isotope Thiamine
chloride (Vitamin B1
HCl) (4,5,4-Methyl-13C3,
99%)
(*C3C9H17ClN4OS)
≥ 98% N/A CIL Until
exhausted or
evidence of
contamination
-20 to-30 C
Protect from
light, air and
moisture.
9 Isotope Riboflavin
(Vitamin B2) (13C4,
99%; 15N2, 98%)
(C13*C4H20N2*N2O6)
≥ 98% N/A CIL Until
exhausted or
evidence of
contamination
Room
temperature
away from
light and
moisture
10 Isotope Nicotinamide
(D4, 98%)
(C6D4H2N2O)
≥ 98% N/A CIL Until
exhausted or
evidence of
contamination
Room
temperature
away from
light and
moisture
11 Isotope Nicotinic acid
(D4, 98%) (C6D4HNO2)
≥ 98% N/A CIL Until
exhausted or
evidence of
contamination
Room
temperature
away from
light and
moisture
12 Isotope Pantothenic
acid, Calcium salt
monohydrate
(beta-alanyl-13C3, 99%;
15N, 98%)
≥ 98% N/A CIL Until
exhausted or
evidence of
contamination
-5 to 5C and
desiccated.
Protect from
light.
13 Isotope Pyridoxine HCl
(4,5-BIS(hydroxyl
methyl)-13C4, 99%)
(*C4C4H11NO3.HCl)
≥ 98% N/A CIL Until
exhausted or
evidence of
contamination
-20 to-30 C
Protect from
light, air and
moisture.
14 Isotope Pyridoxal:HCl
(Methyl-D3, 98%)
(C8D3H6NO3.3HCl)
≥ 98% N/A CIL Until
exhausted or
evidence of
contamination
Room temp
away from
light and
moisture
15 Isotope
Pyridoxamine:2HCl
(Vitamin B6) (Methyl-D3,
≥ 98% N/A CIL Until
exhausted or
evidence of
-20 C Protect
from light.
BVit-01
FOR ERP USE ONLY
DO NOT DISTRIBUTE