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Simultaneous Determination of Thiamine HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide),

Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant

Formula and Adult Nutritionals

CONFIDENTIAL METHOD FOR ERP ASSESSMENT ONLY

PAGE 2 OF 13

CHEMICALS & REAGENTS

Equivalents may be used unless otherwise specified. Standard and reagent preparations may be scaled

up or down as needed.

No. Reagent (specify grade,

if applicable)

Concentration Preparation

No.

Brand

Shelf life

Storage

1

Formic acid (CH

2

O

2

), AR

98%

N/A

Merck as per COA

Room

temperature

2

Hydrochloric Acid (HCl),

AR

37%

N/A

Merck as per COA

Room

temperature

3

Hepafluorobutyric acid

(HFBA)

98%

N/A

Sigma as per COA

Room

temperature

4

Methanol (MeOH;

CH

3

OH), HPLC Grade

100%

N/A

J.T.

Baker

as per COA

Room

temperature

5

Acetronitrile (ACN;

CH

3

CN)

100%

N/A

J.T.

Baker

as per COA

Room

temperature

6

Ammonium formate, AR

≥ 99%

N/A

Fluka

as per COA

2-8

C

7

Ammonium hydroxide

(NH

4

OH), AR

28-30%

N/A

Merck as per COA

Room

temperature

8

Isotope Thiamine

chloride (Vitamin B1

HCl) (4,5,4-Methyl-13C3,

99%)

(*C3C9H17ClN4OS)

≥ 98%

N/A

CIL

Until

exhausted or

evidence of

contamination

-20 to-30

C

Protect from

light, air and

moisture.

9

Isotope Riboflavin

(Vitamin B2) (13C4,

99%; 15N2, 98%)

(C13*C4H20N2*N2O6)

≥ 98%

N/A

CIL

Until

exhausted or

evidence of

contamination

Room

temperature

away from

light and

moisture

10

Isotope Nicotinamide

(D4, 98%)

(C6D4H2N2O)

≥ 98%

N/A

CIL

Until

exhausted or

evidence of

contamination

Room

temperature

away from

light and

moisture

11

Isotope Nicotinic acid

(D4, 98%) (C6D4HNO2)

≥ 98%

N/A

CIL

Until

exhausted or

evidence of

contamination

Room

temperature

away from

light and

moisture

12

Isotope Pantothenic

acid, Calcium salt

monohydrate

(beta-alanyl-13C3, 99%;

15N, 98%)

≥ 98%

N/A

CIL

Until

exhausted or

evidence of

contamination

-5 to 5

C and

desiccated.

Protect from

light.

13

Isotope Pyridoxine HCl

(4,5-BIS(hydroxyl

methyl)-13C4, 99%)

(*C4C4H11NO3.HCl)

≥ 98%

N/A

CIL

Until

exhausted or

evidence of

contamination

-20 to-30

C

Protect from

light, air and

moisture.

14

Isotope Pyridoxal:HCl

(Methyl-D3, 98%)

(C8D3H6NO3.3HCl)

≥ 98%

N/A

CIL

Until

exhausted or

evidence of

contamination

Room temp

away from

light and

moisture

15

Isotope

Pyridoxamine:2HCl

(Vitamin B6) (Methyl-D3,

≥ 98%

N/A

CIL

Until

exhausted or

evidence of

-20

C Protect

from light.

BVit-01

FOR ERP USE ONLY

DO NOT DISTRIBUTE