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Simultaneous Determination of Thiamine HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide),
Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant
Formula and Adult Nutritionals
CONFIDENTIAL METHOD FOR ERP ASSESSMENT ONLY
PAGE 3 OF 13
98%)
(C8D3H9N2O2.2HCl)
contamination
16
Isotope Biotin (chemical
purity 97%) (6,6-D2,
98%)
(C10D2H14N2O3S)
97%
N/A
CIL
Until
exhausted or
evidence of
contamination
Room temp
away from
light and
moisture
17
Thiamine Hydrochloride
(Vitamin B1 )
(C12H17ClN4OS-HCl)
99.8%
N/A
USP
See remark
below
2-8
C
18
Riboflavin (Vitamin B2)
(C17H2ON4O6)
100 %
N/A
USP
See remark
below
2-8
C
19
Nicotinamide
(C6H6N2O), USP
99.9%
N/A
USP
See remark
below
2-8
C
20
Nicotinic acid
(C6H5NO2)
99.8%
N/A
USP
See remark
below
2-8
C
21
Calcium pantothenate
(C18H32CaN2O10)
99%
N/A
USP
See remark
below
2-8
C
22
Pyridoxine hydrochloride
(C8H11NO3. HCl)
99.5%
N/A
USP
See remark
below
2-8
C
23
Pyridoxal hydrochloride
(C8H9NO3.HCl)
≥ 99 %
N/A
Sigma-
Aldrich
as per COA
-20 to-30
C
24
Pyridoxamine
dihidrochloride
(C8H12N2O2. 2HCl)
≥ 98 %
N/A
Sigma-
Aldrich
as per COA
-20 to-30
C
25
Biotin (C10H16N2O3S)
99.3%
N/A
USP
See remark
below
2-8
C
26
Deionized water (DI)
Resistivity ≥ 18.2 M
Fresh
27
75 mM Formic acid-20%
MeOH mixture solution
1
1 year
Room
temperature
28
300 mM Formic acid-
20% MeOH mixture
solution
2
1 year
Room
temperature
28
1 N HCl
3
1 year
Room
temperature
29
50 mM HCl
4
1 year
Room
temperature
30
1 % Formic acid
5
1 year
Room
temperature
31
Thiamine Cl (Vitamin
B1) stock internal
standard solution
20 mcg/ml
6
Until
exhausted or
evidence of
contamination
2-8
C
32
Riboflavin (Vitamin B2)
stock internal standard
solution
20 mcg/ml
7
Until
exhausted or
evidence of
contamination
2-8
C
33
Nicotinamide stock
internal standard
solution
270 mcg/ml
8
Until
exhausted or
evidence of
contamination
2-8
C
34
Nicotinic acid stock
internal standard
solution
25 mcg/ml
9
Until
exhausted or
evidence of
2-8
C
BVit-01
FOR ERP USE ONLY
DO NOT DISTRIBUTE