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Simultaneous Determination of Thiamine HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide),

Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant

Formula and Adult Nutritionals

CONFIDENTIAL METHOD FOR ERP ASSESSMENT ONLY

PAGE 3 OF 13

98%)

(C8D3H9N2O2.2HCl)

contamination

16

Isotope Biotin (chemical

purity 97%) (6,6-D2,

98%)

(C10D2H14N2O3S)

97%

N/A

CIL

Until

exhausted or

evidence of

contamination

Room temp

away from

light and

moisture

17

Thiamine Hydrochloride

(Vitamin B1 )

(C12H17ClN4OS-HCl)

99.8%

N/A

USP

See remark

below

2-8

C

18

Riboflavin (Vitamin B2)

(C17H2ON4O6)

100 %

N/A

USP

See remark

below

2-8

C

19

Nicotinamide

(C6H6N2O), USP

99.9%

N/A

USP

See remark

below

2-8

C

20

Nicotinic acid

(C6H5NO2)

99.8%

N/A

USP

See remark

below

2-8

C

21

Calcium pantothenate

(C18H32CaN2O10)

99%

N/A

USP

See remark

below

2-8

C

22

Pyridoxine hydrochloride

(C8H11NO3. HCl)

99.5%

N/A

USP

See remark

below

2-8

C

23

Pyridoxal hydrochloride

(C8H9NO3.HCl)

≥ 99 %

N/A

Sigma-

Aldrich

as per COA

-20 to-30

C

24

Pyridoxamine

dihidrochloride

(C8H12N2O2. 2HCl)

≥ 98 %

N/A

Sigma-

Aldrich

as per COA

-20 to-30

C

25

Biotin (C10H16N2O3S)

99.3%

N/A

USP

See remark

below

2-8

C

26

Deionized water (DI)

Resistivity ≥ 18.2 M

Fresh

27

75 mM Formic acid-20%

MeOH mixture solution

1

1 year

Room

temperature

28

300 mM Formic acid-

20% MeOH mixture

solution

2

1 year

Room

temperature

28

1 N HCl

3

1 year

Room

temperature

29

50 mM HCl

4

1 year

Room

temperature

30

1 % Formic acid

5

1 year

Room

temperature

31

Thiamine Cl (Vitamin

B1) stock internal

standard solution

20 mcg/ml

6

Until

exhausted or

evidence of

contamination

2-8

C

32

Riboflavin (Vitamin B2)

stock internal standard

solution

20 mcg/ml

7

Until

exhausted or

evidence of

contamination

2-8

C

33

Nicotinamide stock

internal standard

solution

270 mcg/ml

8

Until

exhausted or

evidence of

contamination

2-8

C

34

Nicotinic acid stock

internal standard

solution

25 mcg/ml

9

Until

exhausted or

evidence of

2-8

C

BVit-01

FOR ERP USE ONLY

DO NOT DISTRIBUTE