417 / 620
Parameter
Method Performance
Please
report in units as stated in SMPR!
Weighting
factor for
parameter
Suitability Ranking (1-
3-5)
(select from drop-down
list,5=best)
SPIFANmatrices
Applicable to all formsof infant,adult,and/orpediatric
formula (powders, ready-to-feed liquids,and liquid
concentrates).
Any combinationofmilk, soy, rice,whey,hydrolyzed
protein, starch,andamino acids,withandwithout intact
protein.
MLT included several foodmatrices, among
which1milkpowderbased infant formula.Data
see JAOAC Int95, 1:2012:1. repeatability data
taken from this study.
Reproducibility datacalculated fromP-testdata
involving 30 laboratoriesusing AOAC 2011.14on
5 infant/adultnutritional formulamatrices:
infant formulapowdermilkbased, infant
formulapowderpartially hydrolyzedmilkbased,
adultnutritionalRTF, adultnutritionalRTFhigh
protein, peadiatricnutritionalRTF.See additional
report including individualdata.
1
Allanalytesdefined in the
applicability statementare
measured.
1
Ca
20-1280
2-300
Cu
0.001-1.2
0.02-2.7
Fe
0.01-20
0.1-11
Mg
3-110
1-94
P
15-800
2-200
Na
10-850
2-200
K
20-1280
2.5-400
Mn
0.001-1
0.001-0.25
Zn
0.1-18
0.1-4
Analyte1
Analyte2
Analyte3
Analyte4
Analyte5
Ca
15
2mg/100g reconstitutedproduct
Cu
0.001
0.02mg/100g reconstitutedproduct
Fe
0.01
0.1mg/100g reconstitutedproduct
K
10
2.5mg/100g reconstitutedprodcut
Mn
0.001
0.001mg/100g reconstitutedproduct
Na
10
2mg/100g reconstitutedproduct
P
15
2mg/100g reconstitutedproduct
Zn
0.1
0.1mg/100g reconstitutedproduct
Mg
3
1mg/100g reconstitutedproduct
Spike recovery (%)
2
BiasvsSRM 90-110%
Recovery calculated from P-testdataand
expressedas foundvalueversusassignedvalue:
range forall9minerals88.8-103.2%
3
Ca
5%
1.34
Cu
5%
3.06
Fe
5%
3.02
K
5%
1.01
Mg
5%
1.27
Mn
8%
2.56
Na
5%
1.05
P
5%
1.24
Zn
5%
2.66
Ca
8%
2.1-4.2
Cu
10%
2.9-9.0
Fe
10%
1.6-6.8
Mg
8%
0.9-3.1
P
8%
1.9-4.2
K
8%
2.0-6.2
Na
8%
1.8-3.1
Zn
10%
1.4-4.6
Mn
16%
2.1-14.3
b
Units
Limitof quantification (LOQ)
Method Reference #
AOAC 2011.14
Method title:
Principle of the method:
Determination of Ca, Cu, Fe, Mg, MN, K, P, Na, Zn in fortified food products by microwave
digestion and ICP-OES
ICP-OES determination of 9 minerals
Notes:
Overall Score
Intermediate Reproducibility
(RSDiR)
Recommendation of ERP
2 years after First Action Status
Evaluation of Method Performance vs. SMPR requirements.
Feedback fromUsersof the Method since being awarded
FirstAction
OfficialMethods
Status
Repeatability (RSDr)
Reproducibility (RSDR)
Accuracy/Recovery
ADDITIONAL EVALUATION PARAMETERS
SMPR Requirement
AnalyticalRange.
1
AOAC SMPR: 2014.004
Limitof detection (LOD)
1
a
Concentrationsapply to (1) "ready to feed liquids" "as-is"; (2) reconstitutedpowders (25g into200gwater);and (3) liquid concentratesdiluted1:1by weight.
DidMethodAuthorConsiderERP’sMethodSpecific
Recommendations
(Seeweb link to specificmethod
comments):
Decision by ERP
Proprietary equipment
Laboratory safety
OtherConsiderations
move to FinalAction/repeal/remove/expand2year term
0
Nounique proprietary equipment/accessoriesare required.
Methoddoesnot require any special safety precautionse.g.personalprotection fromhighly toxic solvents.
3
1
2
Biasagainstestablishedmethod
Analyticalequipment
1
Adequate proofof performance via system suitability
Is there abiasYes/No?
Analyticalequipment is commonlyavailable inmost labs.
SPIFANERPChecklistv1.6
27.06.2013
2011.14 (MTE-01) - MEF FORM
FOR ERP USE ONLY
DO NOT DISTRIBUTE