Protein Determination
SDS‐PAGE protein profile
Trypsin digestion‐derived
d
1
2
Sentandreu
et al.
,
2010
,
9
, 3374‐3383,
J. Proteome Res.
F
F
F
C
C
C
F: fresh;C: cooked
Myosinlightchain3
pepti e sequence
DQGTFEDFVEGLR
EAFLLFDRTGDAK
ILGNPSKEEMNAK
ALGQNPTNAEINK
MTEEEVEELMK
EEMNAKK
3
Fragmentation pattern
4
Absolute quantitation by LC‐MS/MS
100
0
Time (min)
Relative abundance
t
R
= 84.02 min
Meat Species Substitution - Proteomics
+
High discriminating power
►
Within-species tissue differentiation
Extraction protocols ‘easy’ to standardize
Primary amino acid sequence of peptides resistant to processing
►
►
►
‐
High cost of modern mass spectrometry equipments
Need for skilled operators
►
►
