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TLA Panel Allergens: priorities and challenges for food allergen detection
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MPR for gluten: CEN activities (III)
- Structure follows CEN requirements but also contains important AOAC SMPR elements
-
Title
: Minimum Performance Requirements for quantitative gluten measurement by
ELISA
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Scope
: …specifies minimum requirements on information and performance for
immunological methods that quantify intact or fragmented gluten from wheat, rye, and
barley in raw and processed foodstuffs. Information on applicability, antibody,
construction of the test system, calibration material, curve fitting algorithm, and
reporting of results shall be given by the method provider. Performance characteristics
that shall be validated for each method are specificity, selectivity, limit of detection,
limit of quantification, cross reactivity, interfering substances, recovery, precision,
accuracy, trueness, and robustness.
TLA Panel Allergens: priorities and challenges for food allergen detection
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MPR for gluten: CEN activities (IV)
- Normative References
- Terms and Definitions: gluten, prolamin, exception for oats
- General information on the test system
- Performance criteria (how to determine…..)
- Overview on Minimum Performance Requirements
- Table 1: Intact gluten
- Table 2: fragmented gluten
Parameter
Gluten
Analytical Range
[mg/kg]
10 to 40
LOQ [mg/kg]
10
LOD [mg/kg]
5
Recovery [%]
70 to 130
RSD
r
[%]
15
RSD
R
[%]
30
Specificity
Gluten from wheat, rye, and barley