Methods to be Reviewed by OMB in 2016

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1394 Jaudzems: Journal of AOAC International Vol. 98, No. 5, 2015
The analytical range for SPIFAN samples was found to be
between 1.4 and 1060 mg/100 g reconstituted product or RTF.
The LOQ was estimated as 1.4 mg/100 g. Results of precision
studies for chloride expressed on reconstituted products are
presented in Table 2015.08A . RSDr ranged from 0.03 to 1.60%
in the range from 20 to 167 mg/100 g RTF, and RSDR ranged
from 0.09 to 2.77% over the same range, thus fulfilling the
≤ 2 and 4% performance requirements, respectively. All samples
met the intermediate reproducibility (RSD R) performance
requirement except for one RTF sample with high fat content,
which gave a precision value of 10.09%. This RTF sample was
problematic due to heterogeneity and separation since it was
being used beyond its recommended shelf life.
Accuracy was proven by analyzing SRM 1849a in duplicate
on 6 different days and comparing the overall mean to the SRM
informational value (not certified; Table 2015.08B). The overall
mean was 68.5 mg/100 g for the reconstituted product with
an RSDR of 1.59%, equivalent to a recovery of 97.2%. Spike
recovery was performed on 15 different SPIFAN products
(powder and liquid infant formula and adult nutritionals). The
reconstituted powders and RTF products were spiked at two
different levels (50 and 100% of native chloride content) and
analyzed in duplicate on 6 different days (Table 2015.08B).
Spike recovery (99–104%) was within the tolerance (95–105%)
defined in the SMPR.
All results compared to SMPR values are shown in
Table 2015.08C . All results were within the target values
defined in AOAC SMPR 2014.015.
Conclusions
The data presented in this paper were submitted to the AOAC
ERP for review at the AOAC INTERNATIONAL Mid-Year
Meeting held on March 17, 2015. The ERP determined that
the data presented were in accordance with SMPR 2014.015
approved by SPIFAN, and the method was granted First Action
status.
References
(1) AOAC SMPR 2014.015,
org/10.5740/jaoacint.SMPR2014.016
(2) Sullivan, D. (2012) J. AOAC Int. 95, 278–297.
10.5740/jaoacint.Sullivan_Intro
(3) Official Methods of Analysis (2012) 19th Ed., AOAC
INTERNATIONAL, Rockville, MD, Appendix L: AOAC
Recommended Guidelines for Stakeholder Panel on Infant
Formula and Adult Nutritionals (SPIFAN) Single-Laboratory
Validation,
Candidates for 2016 Method of the Year
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