Table of Contents Table of Contents
Previous Page  318 / 407 Next Page
Information
Show Menu
Previous Page 318 / 407 Next Page
Page Background

AOAC Final Action Method 2016.02 (Biotin)

Page 12 of 16

Table 2016.02G: Statistical evaluation biotin results from SPIFAN matrices

Samples

Product Description

p

Mean %RSD

r

%RSD

R

HorRat

Practice 1

Infant Formula Powder Partially Hydrolysed Soy Based

12

38.59

3.18

6.98

0.38

Practice 2

Infant Formula Powder FOS/GOS Based

12

14.94

4.92

7.74

0.36

MLT - 1

Infant Formula Powder Partially Hydrolysed Milk Based

9

34.01

5.53

9.48

0.50

MLT - 2

Infant Elemental Powder

9

79.20

2.64

5.14

0.31

MLT - 3

Infant Formula RTF Milk Based

9

4.07

5.52

7.59

0.29

MLT - 4

Adult Nutritional RTF High Fat

9

71.46

3.88

7.75

0.46

MLT - 5

Infant Formula Powder Milk Based

9

26.97

6.79

8.76

0.45

MLT - 6

Infant Formula Powder Soy Based

9

44.75

5.77

6.96

0.39

MLT - 7

NIST SRM 1849a

9

196.5

2.38

3.04

0.21

MLT - 8

Adult Nutritional Powder Low Fat

9

258.7

2.81

7.03

0.51

MLT - 9

Child Formula Powder

9

166.8

7.03

7.03

0.47

MLT - 10

Toddler Formula Powder Milk-Based

9

10.12

5.74

6.65

0.29

MLT - 11

Infant Formula Powder Milk Based

9

42.90

2.26

5.72

0.31

MLT - 12

Adult Nutritional RTF High Protein

9

52.68

5.14

5.89

0.33

p = total number of laboratories

RSD

r

= relative standard deviation of repeatability

RSD

R

= relative standard deviation of reproducibility

HorRat = Horwitz Ratio (RSD

R

/ PRSD

R

)

Table 2016.02H: Comparison with LCMS/MS technique

Sample # Product Description

Blind

Duplicates

Biotin (µg/100g)

AOAC 2016.02

LCMS/MS

Difference

MLT 1

Infant Formula Powder Partially

Hydrolysed Milk Based

A

33.78

35.55

-1.77

B

34.23

39.10

-4.87

MLT 2

Infant Elemental Powder

A

78.79

82.45

-3.66

B

79.61

81.15

-1.54

MLT 3

Infant Formula RTF Milk Based

A

4.02

3.73

0.29

B

4.13

3.84

0.29

MLT 4

Adult Nutritional RTF High Fat

A

71.90

66.06

5.84

B

71.02

77.49

-6.47

MLT 5

Infant Formula Powder Milk Based

A

26.67

26.99

-0.32

B

27.27

25.25

2.02

MLT 6

Infant Formula Powder Soy Based

A

44.36

43.00

1.36

B

45.14

42.27

2.87

MLT 7

NIST SRM 1849a

A

196.06

186.58

9.48

B

196.96

181.50

15.46

MLT 8

Adult Nutritional Powder Low Fat

A

260.88

263.90

-3.02

B

256.49

246.17

10.32

MLT 9

Child Formula Powder

A

174.60

171.69

2.91

B

159.09

162.77

-3.68

MLT 10

Toddler Formula Powder Milk-Based

A

10.38

9.87

0.51

B

9.85

10.30

-0.45

MLT 11

Infant Formula Powder Milk Based

A

42.84

41.66

1.18

B

42.96

39.77

3.19

MLT 12

Adult Nutritional RTF High Protein

A

52.63

54.05

-1.42

B

52.73

51.57

1.16

2016.02 (BIOTIN) FINAL ACTION REVIEW

FOR ERP USE ONLY

DO NOT DISTRIBUTE

210