63 / 120
Liposomes, Exosomes, and Virosomes: From Modeling Complex
Membrane Processes to Medical Diagnostics and Drug Delivery
Poster Abstracts
58
19-POS
Board 10
Monodisperse Multicompartment Liposomes
Nan-Nan Deng
, Wilhelm Huck.
Radboud University, Nijmegen, Netherlands.
Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from
drug delivery to synthetic biology. However, high throughput, controlled fabrication of
monodisperse liposomes with controllable compartments has not been achieved thus far. Here,
we report on a surfactant-assisted microfluidic route to assemble oil-free monodisperse
liposomes with single- or multi-compartments via precise control over interfacial energies of
complex water-in-oil-in-water (W/O/W) emulsion systems. The use of various emulsion
templates, in combination with the spontaneous dewetting afforded by the surfactant, allows for
the creation of uniform, single bilayer liposomes with multiple inner compartments. This method
is compatible with complex bioreactions as exemplified by the production of eGFP in liposomes
via cell-free gene expression. We believe this is the example where liposome formation from
microfluidically prepared double emulsion templates is fully controlled. The high yield, high
throughput, and uniformity of structures produced has potential for future designs of cell-like
bio-devices or as coupled microreactors.