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Liposomes, Exosomes, and Virosomes: From Modeling Complex
Membrane Processes to Medical Diagnostics and Drug Delivery
Poster Abstracts
60
25-POS
Board 13
G Protein-Coupled Receptors Incorporated Into Rehydrated Diblock Copolymer Vesicles
Retain Functionality
Mary Gertrude Gutierrez
1
, Farzad Jalali-Yazdi
1
, Justin Peruzzi
2
, Carson T. Riche
1
, Richard W.
Roberts
1
, Noah Malmstadt
1
.
1
University of Southern California, Los Angeles, USA,
2
University of Virginia, Charlottesville,
VA, USA.
We successfully incorporated the human serotonin receptor, G Protein Coupled Receptor
(GPCR), 5-HT
1A
R, in polymeric giant unilamellar protein-vesicles (pGUPs) made of diblock
copolymer. By utilizing an agarose rehydration technique for protein incorporation, the GPCR is
inserted in the biased, physiological orientation with the C-terminus cytosolic and N-terminus
extracellular. The GPCR is fully functional within the polymeric bilayer, exhibiting
physiological responses to various ligands. The entire population of GPCRs in pGUPs remains
fully functional after lyophilization for 120 hours.