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Document 1 of 2 – 3M Petrifilm Salmonella Express PTM Study Report
Page 1 of 41
NOTE:
The official document is electronic. Paper copies are uncontrolled.
Evaluation of the 3M
™
Petrifilm™ Salmonella Express System for the Detection of
Salmonella in Select Foods
AOAC Research Institute
Performance Tested Method
Abstract
The 3M™ Petrifilm™ Salmonella Express SALX System is used for the rapid and specific detection and l biochemical confirmation of
Salmonella
spp.
from enriched food matrices. The 3M Petrifilm SALX System was compared to the USDA/FSIS-MLG 4.05
Isolation and
Identification of Salmonella from Meat, Poultry, Pasteurized Egg and Catfish Products
for raw ground chicken, pasteurized liquid whole
egg, raw ground beef, raw ground pork, and cooked chicken nuggets and to FDA/BAM Chapter 5
Salmonella
for frozen uncooked
shrimp, fresh bunched spinach, dry dog food, and stainless steel. Twenty (20) replicates of each matrix were analyzed at one inoculum
level: 0.2-2 CFU/test portion. Five (5) control replicates were also analyzed at 0 CFU/test portion (un-inoculated). Results were
analyzed by both the Mantel-Haenszel chi square for unpaired test portions and by the Probability of Detection (POD). There were no
statistically significant differences in the number of positive samples detected and confirmed by the 3M Petrifilm SALX
System and the
reference methods for the nine matrices analyzed. Lot-to-lot assay variability, shelf-life stability, and assay ruggedness parameters
(primary enrichment incubation time, incubation time of the 3M™ Petrifilm™ Salmonella Plate, incubation temperature of the 3M™
Petrifilm™ Salmonella Plate, incubation temperature of the 3M™ Petrifilm™ Salmonella Disk, incubation time of the 3M™ Petrifilm™
Salmonella Disk, and various plate rehydration options were evaluated and found no unexpected results. The 3M™ Petrifilm™ SALX
System demonstrated reliability as a rapid qualitative method for the detection and biochemical confirmation of
Salmonella
in a wide
variety of foods.
Method Author
Robert P. Jechorek
3M Food Safety Department
3M Center - Bldg 260-6B-01
St. Paul, MN 55144-1000
Submitting Company
3M Company
Food Safety Department
3M Center,
Bldg. 275-5W-05