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Document 1 of 2 – 3M Petrifilm Salmonella Express PTM Study Report
Page 6 of 41
NOTE:
The official document is electronic. Paper copies are uncontrolled.
2.
3M
Salmonella
Enrichment Supplement
3.
3M Petrifilm
Salmonella
Express (SALX) Plate
4.
3M Petrifilm
Salmonella
Express (SALX) Confirmation Disk
Additional Supplies and Reagents available from 3M Food Safety, St. Paul, MN
1.
3M Petrifilm Flat Spreader
2.
3M Rappaport-Vassiliadis R10 (R-VR10) Broth
Additional Supplies and Reagents required but not provided
1.
Sterile 10µL inoculation loop. -
for streaking enrichments onto the SALX plate
2.
Permanent ultra fine tip marker. –
for circling presumptive positive colonies on the SALX plate
3.
Sterile serological pipettes. –
capable of 1mL
4.
Pipettes
. – capable of 100µL
5.
Sterile pipette tips.
– capable of 100µL
6.
Stomacher®
. – Seward or equivalent
7.
Filter Stomacher® bags
. - Seward or equivalent
8.
Incubators
. – capable of maintaining 41.5° ± 1°C
9.
Freezer
. – capable of maintaining -10° to -20°C, for storing opened SALX pouches , hydrated SALX plates, SALX plates after
incubation
10.
Refrigerator
. – capable of maintaining 2° - 8°C, for storing unopened 3M SALX plates and SALX disks
11.
Sterile distilled water
12.Sterile reverse osmosis water
13.Sterile Butterfield’s Phosphate Diluent
Safety Precautions
The 3M Petrifilm SALX System is intended for use in a laboratory environment by professionals trained in laboratory techniques. 3M has not
documented the use of this product in industries other than food. For example, 3M has not documented this product for testing water,
pharmaceutical, cosmetic, clinical or veterinary samples. The 3M Petrifilm SALX System has not been evaluated with all possible food
products, food processes and food processing environments, testing protocols or with all possible strains of bacteria and may not detect all
Salmonella
strains. 3M has not validated the 3M Petrifilm SALX System using composite samples.
Do not use the 3M Petrifilm SALX System in the diagnosis of conditions in humans or animals.