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Document 1 of 2 – 3M Petrifilm Salmonella Express PTM Study Report

Page 8 of 41

NOTE:

The official document is electronic. Paper copies are uncontrolled.

The recommended size of the sampling area to verify the presence or absence of the pathogen on the surface is 100 cm

2

(10 cm x 10 cm

or 4 in. x 4 in.)

4

. When sampling with a sponge, cover the entire area going in two directions (left to right then up and down).

1.

.

2.

If the environmental sample has a high microbial load (total aerobic colony count of > 10

4

colony forming units/sample), then after

primary enrichment incubation, transfer 0.1 mL of the primary enrichment into 10.0 mL Rappaport-Vassiliadis R10 (R-V R10) Broth.

Incubate at 41.5 ± 1.0°C for 8-24 hours.

Table 1:

Sample Enrichment Protocols

TEST MATRIX

H

IGH

M

ICROBIAL

LOAD

(≥10

4

CFU/

G

)

SAMPLE

SIZE

PRIMARY ENRICHMENT

SECONDARY ENRICHMENT

ENRICHMENT

VOLUME

(

M

L)

Temperature

(±1.0°C)

Enrichment

Time (hr)

ENRICHMENT

MEDIUM

Temperature

(±1.0°C)

Enrichment

Time (hr)

Foods: Raw

Meat, Poultry

25 g

225

41.5

18-24

R-V R10 Broth :

0.1 mL into

10.0 mL

41.5

8-24

Foods:

Seafood and

Fish

25 g

225

R-V R10 Broth :

0.1 mL into

10.0 mL

41.5

8-24

Foods:

Pasteurized

Eggs

100 g

900

Not required for low microbial load foods.

Animal Feed

375 g

3375

Not required for low microbial load foods.