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Document 2 of 2 – 3M Petrifilm Salmonella Express PTM Study Report
Table 20. 3M Petrifilm SALX Method Comparison POD Results for Raw Ground Chicken (Internal Data)
1
x
c
POD
C
d
95% CI
x
POD
R
e
95% CI
Raw
Ground
Chicken
18 Hr
Primary
Enrichment
–
Salmonella
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
Vs.
USDA/FSIS-
MLG
8 Hr 2
0
RV-R10
Enrichment Heidelberg
1.15 (0.70-
2.07)
20
14
0.7 0.48 - 0.85 14
0.7 0.48 - 0.85
0 -0.27 – 0.27
18 Hr
Primary
Enrichment
–
NCTC
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
24 Hr
2
0
RV-R10
Enrichment
5717
1.15 (0.70-
2.07)
20
14
0.7 0.48 - 0.85 14
0.7 0.48 - 0.85
0 -0.27 – 0.27
24 Hr
Primary
Enrichment
–
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
8 Hr 2
0
RV-R10
Enrichment
1.15 (0.70-
2.07)
20
14
0.7 0.48 - 0.85 14
0.7 0.48 - 0.85
0 -0.27 – 0.27
24 Hr
Primary
Enrichment
–
0
5
0
0
0.00 - 0.43
0
0
0.00 - 0.43
0 -0.43 – 0.43
24 Hr
2
0
RV-R10
Enrichment
1.15 (0.70-
2.07)
20
14
0.7 0.48 - 0.85 14
0.7 0.48 - 0.85
0 -0.27 – 0.27
a
MPN = Most Probable Number is based on the POD of reference method test portions across labs using the AOAC MPN calculator with 95% confidence interval
b
N = Number of test potions
c
x = Number of positive test portions
d
POD
C
=
Candidate method confirmed positive outcomes divided by the total number of trials
e
POD
R
= Reference method confirmed positive outcomes divided by the total number of trials
f
dPOD
C
= Difference between the candidate method confirmed results and candidate method confirmed result POD values
Reference Method
dPOD
C
f
95% CI
g
g
95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level
Matrix Time Point
Strain MPN
a
/25g
N
b
3M Petrifilm SALX Method
2