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Document 2 of 2 – 3M Petrifilm Salmonella Express PTM Study Report

Table 21. 3M Petrifilm SALX Method Comparison POD Results for Raw Frozen Shrimp (Internal Data)

1

x

c

POD

CP

d

95% CI

x

POD

CC

e

95% CI

Frozen

Uncooked

Shrimp -

18 Hr

Primary

Enrichment

Salmonella

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

8 Hr

2

0

RV-R10

Enrichment Virchow

0.77 (0.41-

1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

Confirmed by

the 3M

Confirmation

Disk

18 Hr

Primary

Enrichment

ATCC

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

24 Hr

2

0

RV-R10

Enrichment

51955

0.77 (0.41-

1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

24 Hr

Primary

Enrichment

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

8 Hr 2

0

RV-

R10

Enrichment

0.77 (0.41-

1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

24 Hr

Primary

Enrichment

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

24 Hr 2

0

RV-R10

Enrichment

0.77 (0.41-

1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

Frozen

Uncooked

Shrimp -

18 Hr

Primary

Enrichment

Salmonella

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

8 Hr 2

0

RV-R10

Enrichment Virchow

0.77 (0.41-

1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

Confirmed by

the Reference

Method

18 Hr

Primary

Enrichment

ATCC

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

24 Hr 2

0

RV-R10

Enrichment

51955

0.77 (0.41-

1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

24 Hr

Primary

Enrichment

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

8 Hr 2

0

RV-R10

Enrichment

0.77 (0.41-

1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

24 Hr

Primary

Enrichment

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

24 Hr 2

0

RV-R10

Enrichment

0.77 (0.41-

1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

d

POD

C

=

Candidate method confirmed positive outcomes divided by the total number of trials

e

POD

R

= Reference method confirmed positive outcomes divided by the total number of trials

f

dPOD

C

= Difference between the candidate method confirmed results and candidate method confirmed result POD values

g

95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level

Reference Method

dPOD

CP

f

95% CI

g

a

MPN = Most Probable Number is based on the POD of reference method test portions across labs using the AOAC MPN calculator with 95% confidence interval

b

N = Number of test potions

c

x = Number of positive test portions

Matrix

Time Point

Strain MPN

a

/25g

N

b

3M Petrifilm SALX Method

2