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Table 30.

3M Petrifilm RYM Method Compared to ISO 21527:2008 part 1 and

1

FDA-BAM Chapter 18 for frozen Ground Beef Patties at 48 and 60

2

Hours.

Reference Method

Level

Replicate

cfu/g log cfu/g cfu/g log cfu/g cfu/g log cfu/g

1

<10

<1

<10

<1

<10

<1

2

<10

<1

<10

<1

<10

<1

3

<10

<1

<10

<1

<10

<1

4

<10

<1

<10

<1

<10

<1

5

<10

<1

<10

<1

<10

<1

Mean

NA

NA

NA

Sr

NA

NA

NA

RSDr, %

NA

NA

NA

p

-value

NA

NA

NA

1

80

1.9

90

1.95

90

1.95

2

100

2.00

100

2.00

60

1.78

3

70

1.85

70

1.85

60

1.78

4

100

2.00

120

2.08

100

2.00

5

70

1.85

100

2.00

80

1.90

Mean

84

1.92

96

1.98

78

1.88

Sr

0.08

0.09

0.10

RSDr, %

4.16

4.55

5.32

p

-value

0.52

0.60

1

960

2.98

1020

3.01

570

2.76

2

810

2.91

870

2.94

510

2.71

3

780

2.89

820

2.91

680

2.83

4

780

2.89

840

2.92

640

2.81

5

780

2.89

870

2.94

600

2.78

Mean

822

2.91

884

2.95

600

2.78

Sr

0.04

0.04

0.05

RSDr, %

1.37

1.36

1.80

p

-value

0.01

0.01

1

7100

3.85

7000

3.85

5400

3.73

2

6300

3.80

6700

3.83

4400

3.64

3

6600

3.82

6900

3.84

4100

3.61

4

5700

3.76

6000

3.78

3700

3.57

5

5900

3.77

6100

3.79

4100

3.61

Mean

6320

3.8

6540

3.81

4340

3.63

Sr

0.04

0.03

0.06

RSDr, %

1.05

0.79

1.65

p

-value

0.00

0.00

High

RYM Method-48h

RYM Method-60h

Uninoculated

LOW

Medium

3

4

40

AOAC Research Institute

Expert Review Panel Use Only

OMAMAN-16D/ PTM Report

ERP Use Only - December 2014