332 / 350
Lot 1
10-100 cfu/g
Lot 2
100-1000 cfu/g
Lot 3
1000-10,000 cfu/g
Estimate
Yeast/Mold
Count
Total Aerobic
Count
Estimate
Yeast/Mold
Count
Total Aerobic
Count
Estimate
Yeast/Mold
Count
Total Aerobic
Count
Petrifilm
RYM
ISO&BAM
Inoculate
Petrifilm RYM
with 1.0 ml of
each dilution in
duplicate,
spread with 3M
spreader
Homogenize 25g+225mL
0.1% peptone water
Serially dilute each of 5
samples 10
-1
to 10
-6
in 0.1%
peptone water
Spread 0.1 ml of
each dilution in
triplicate on
DRBC
Incubate
48±2hr @
25±1C, if
colonies faint,
incubate
another 12 hr
Incubate 5-7
days @ 25C
Record colony
count of yeast
and mold
separately
Record colony
count of yeast
and mold
separately
Petrifilm
RYM
Incubate 5-7
days @ 25C
Record colony
count of yeast
and mold
separately
Record colony
count of yeast
and mold
separately
Petrifilm
RYM
Incubate 5-7
days @ 25C
Record colony
count of yeast
and mold
separately
Record colony
count of yeast
and mold
separately
Homogenize 25g+225mL
0.1% peptone water
Serially dilute each of 5
samples 10
-1
to 10
-6
in 0.1%
peptone water
Homogenize 25g+225mL
0.1% peptone water
Serially dilute each of 5
samples 10
-1
to 10
-6
in 0.1%
peptone water
Incubate
48±2hr @
25±1C, if
colonies faint,
incubate
another 12 hr
Incubate
48±2hr @
25±1C, if
colonies faint,
incubate
another 12 hr
ISO&BAM
ISO&BAM
Inoculate
Petrifilm RYM
with 1.0 ml of
each dilution in
duplicate,
spread with 3M
spreader
Inoculate
Petrifilm RYM
with 1.0 ml of
each dilution in
duplicate,
spread with 3M
spreader
Spread 0.1 ml of
each dilution in
triplicate on
DRBC
Spread 0.1 ml of
each dilution in
triplicate on
DRBC
1
2
3
4
5
6
7
8
9
10
11
M
ETHOD
C
OMPARISON
T
ESTING
12
36
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-16D/ PTM Report
ERP Use Only - December 2014