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No statistically significant difference was observed between all 3 levels analyzed between the two

1

methods. The p-values calculated at a 95% confidence level for the low and medium levels and high

2

levels were 0.1461, 0.7194, and 0.0837, respectively. The t-test indicated no significant differences

3

between the 3M RYM method and the ISO and FDA BAM methods. The ISO/FDA BAM method had a

4

lower repeatability value than the 3M RYM method for all levels, with S

r

values, respectively, of

5

0.4273and 0.5072 for the low level and 0.0554 and 0.0686 for the medium level, and 0.0481 and 0.0352

6

for the high level. Detailed results are presented in Table 26.

7

8

9

10

11

12

Table 26.

Method Comparison Results for Fermented Salami at 60 Hours.

13

SD

SD

(S

r

)

(S

r

)

1

<10

<0

<10

<0

2

<10

<0

<10

<0

3

<10

<0

<10

<0

4

<10

<0

<10

<0

5

<10

<0

<10

<0

1

<10

<0

<10

<0

2

1.1 x10

2

2.0414

1.0x10

2

2.0000

3

1.0x10

1

1.0000

2.0 x10

1

1.3010

4

1.0x10

1

1.0000

1.0 x10

1

1.0000

5

4.0x10

1

1.6021

4.0x10

1

1.6021

1

3.2 x10

2

2.5051

3.4x10

2

2.5315

2

3.6 x10

2

2.5563

3.4x10

2

2.5315

3

3.9 x10

2

2.5911

3.2 x10

2

2.5051

4

3.0 x10

2

2.4771

2.5 x10

2

2.3979

5

2.6 x10

2

2.415

3.2x10

2

2.5051

1 2.5 x 10

3

3.3979

3.5 x 10

4

3.301

2

2.6 x 10

3

3.415

4.3 x 10

4

3.3802

3

2.6 x 10

3

3.415

4.9 x 10

4

3.3802

4

2.2 x 10

3

3.3424

4.5x 10

4

3.3424

5

3.0 x 10

3

3.4771

5.2 x 10

4

3.3802

High

3.4095 0.0481

3.3568 0.0352 0.0837

Medium

2.5089 0.0686

2.4942 0.0554 0.7194

-

NA

Low

1.4109 0.5072

1.4758 0.4273 0.1461

Uninoculated

-

-

-

Level

Sample

3M RYM Petrifilm

ISO/FDA BAM

p-value

Ct/g

LOG MEAN

Ct/g

LOG MEAN

14

15

Frozen Ground Beef Patties 48 hours

16

32

AOAC Research Institute

Expert Review Panel Use Only

OMAMAN-16D/ PTM Report

ERP Use Only - December 2014