No statistically significant difference was observed between all 3 levels analyzed between the two
1
methods. The p-values calculated at a 95% confidence level for the low and medium levels and high
2
levels were 0.1461, 0.7194, and 0.0837, respectively. The t-test indicated no significant differences
3
between the 3M RYM method and the ISO and FDA BAM methods. The ISO/FDA BAM method had a
4
lower repeatability value than the 3M RYM method for all levels, with S
r
values, respectively, of
5
0.4273and 0.5072 for the low level and 0.0554 and 0.0686 for the medium level, and 0.0481 and 0.0352
6
for the high level. Detailed results are presented in Table 26.
7
8
9
10
11
12
Table 26.
Method Comparison Results for Fermented Salami at 60 Hours.
13
SD
SD
(S
r
)
(S
r
)
1
<10
<0
<10
<0
2
<10
<0
<10
<0
3
<10
<0
<10
<0
4
<10
<0
<10
<0
5
<10
<0
<10
<0
1
<10
<0
<10
<0
2
1.1 x10
2
2.0414
1.0x10
2
2.0000
3
1.0x10
1
1.0000
2.0 x10
1
1.3010
4
1.0x10
1
1.0000
1.0 x10
1
1.0000
5
4.0x10
1
1.6021
4.0x10
1
1.6021
1
3.2 x10
2
2.5051
3.4x10
2
2.5315
2
3.6 x10
2
2.5563
3.4x10
2
2.5315
3
3.9 x10
2
2.5911
3.2 x10
2
2.5051
4
3.0 x10
2
2.4771
2.5 x10
2
2.3979
5
2.6 x10
2
2.415
3.2x10
2
2.5051
1 2.5 x 10
3
3.3979
3.5 x 10
4
3.301
2
2.6 x 10
3
3.415
4.3 x 10
4
3.3802
3
2.6 x 10
3
3.415
4.9 x 10
4
3.3802
4
2.2 x 10
3
3.3424
4.5x 10
4
3.3424
5
3.0 x 10
3
3.4771
5.2 x 10
4
3.3802
High
3.4095 0.0481
3.3568 0.0352 0.0837
Medium
2.5089 0.0686
2.4942 0.0554 0.7194
-
NA
Low
1.4109 0.5072
1.4758 0.4273 0.1461
Uninoculated
-
-
-
Level
Sample
3M RYM Petrifilm
ISO/FDA BAM
p-value
Ct/g
LOG MEAN
Ct/g
LOG MEAN
14
15
Frozen Ground Beef Patties 48 hours
16
32
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-16D/ PTM Report
ERP Use Only - December 2014