326 / 350
logs with a standard deviation of 0.1366. Only one typical colony morphology, large blue colonies with
1
dark centers and diffuse edges, was observed on the 3M RYM plates. That colony morphology matched
2
the morphology of the inoculating organisms,
Paecilomyces
. Further macroscopic and microscopic
3
investigation into the culture verified that it was
Paecilomyces.
Detailed results are in Table 24.
4
5
6
7
8
9
10
Table 24
. Method Comparison Results for Dehydrated Soup at 48 and 60 Hours.
11
SD
SD
(S
r
)
(S
r
)
1
2.0 x10
1
1.301
<10
<0
2
3.0 x10
1
1.4771
<10
<0
3
1.0x10
1
1.0000
<10
<0
4
2.0x10
1
1.301
<10
<0
5
2.0x10
1
1.301
<10
<0
1
3.1 x10
2
2.4914
<10
<0
2
3.4 x10
2
2.5315
<10
<0
3
2.3 x10
2
2.3617
<10
<0
4
3.0 x10
2
2.4771
<10
<0
5
1.8 x10
2
2.2553
<10
<0
1 1.3 x 10
4
3.1139
<10
<0
2
7.2 x 10
4
2.8573
<10
<0
3
9.9 x 10
4
2.9956
<10
<0
4
6.4 x 10
4
2.8062
<10
<0
5
6.3 x 10
4
2.7993
<10
<0
ISO/FDA BAM
p-value
Ct/g
LOG MEAN
Ct/g
LOG MEAN
Low
1.276 0.1721
-
Level
Sample
3M RYM Petrifilm
-
NA
Medium
2.4234 0.1132
-
-
NA
High
2.9145 0.1366
-
-
NA
12
13
Fermented Salami 48 hours
14
No statistical difference was observed between all 3 levels analyzed between the two methods. The p-
15
values calculated at a 95% confidence level for the low, medium, and high levels were 0.7354, 0.7194,
16
and 0.0805, respectively. One outlier was identified by the Cochran and Grubs test for outliers. The t-
17
test indicated no significant differences between the 3M RYM method and the ISO/FDA BAM methods.
18
30
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-16D/ PTM Report
ERP Use Only - December 2014