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Table 20.

Method Comparison Results for Ready-made Pie at 48 Hours.

1

SD

SD

(S

r

)

(S

r

)

1

<10

<0

<10

<0

2

<10

<0

<10

<0

3

<10

<0

<10

<0

4

<10

<0

<10

<0

5

<10

<0

<10

<0

1

2.0x10

1

1.301

1.0x10

1

1.0000

2

1.0x10

1

1.0000

2.0x10

1

1.301

3

1.0x10

1

1.0000

2.0x10

1

1.301

4

1.0x10

1

1.0000

<10

<0

5

2.0x10

1

1.301

<10

<0

1

1.2x10

2

2.0792

1.0 x 10

3

2.0000

2

1.4 x10

2

2.1461

1.4 x 10

3

2.1461

3

1.2 x10

2

2.0792

1.3 x 10

3

2.1139

4

2.1x10

2

2.3222

1.0 x 10

3

2.0000

5

2.5x10

2

2.3979

2.3 x 10

3

2.3617

1 7.2 x 10

3

3.8573

5.0 x 10

4

3.699

2

7.7 x 10

2

3.8865

6.2 x 10

4

3.7924

3

8.0 x 10

3

3.9031

5.8 x 10

4

3.7634

4

7.6 x 10

2

3.8808

4.9 x 10

4

3.6902

5

8.8 x 10

3

3.9445

5.3 x 10

4

3.7243

High

3.8944 0.0324

3.7339 0.0433 0.0001

Medium

2.2049 0.1467

2.1244 0.1482 0.4128

-

NA

Low

1.1204 0.1649

1.

2007 0.1738 0.537

Uninoculated

-

-

-

Level

Sample

3M RYM Petrifilm

ISO/FDA BAM

p-value

Ct/g

LOG MEAN

Ct/g

LOG MEAN

2

Ready-made Pie 60 hours

3

No statistically significant difference was observed between 2 of the 3 levels analyzed between the two

4

methods. The p-values calculated at a 95% confidence level for the low and medium levels were 0.5370

5

and 0.3651. The t-test indicated a significant difference between the 3M RYM method and the ISO and

6

FDA BAM methods on the high level with a p-value of 0.0001. The 3M RYM method had a higher

7

repeatability value than the ISO and FDA BAM methods for the low, medium, and high levels, with S

r

8

values, respectively, of 0.1399 and 0.1482 for the low level and 0.1649 and 0.1482 for the medium level,

9

and 0.0282 and 0.0433 for the high level. Detailed results are presented in Table 21.

10

11

12

13

14

15

27

AOAC Research Institute

Expert Review Panel Use Only

OMAMAN-16D/ PTM Report

ERP Use Only - December 2014